Place the sample to be frozen directly into the slot of the programmed cooling box, close the lid, and place the box in a clean -80°C freezing environment for at least 3 hours without opening it. After cooling is complete, remove the sample and transfer it to liquid nitrogen. For continuous use, open the top and bottom lids of the product at room temperature to allow it to return to room temperature before reuse. It can hold 1ml/2ml cryovials or centrifuge tubes (12-13).